Skip to Main content Skip to Navigation
Journal articles

Quartz Crystal Microbalance Genosensing of Brettanomyces bruxellensis Yeast in Wine Using a Rapid and Efficient Drop and Collect Protocol

Abstract : A miniaturized quartz crystal microbalance (QCM) genosensor is proposed for sensitive and real-time detection of short ssDNA sequences (53 bp) or DNA extracted from Brettanomyces bruxellensis (Brett) yeast cells. The presence of Brett yeast causes a depreciation of the quality of aged fine wines, producing molecules of unpleasant odors and biogenic amines that are harmful to human health. More specifically, standard quartz crystal (S-QCM) and homemade 4 nm gold transmission electron microscopy (TEM)-grid patterned quartz (multi-TEM QCM) are herein proposed for biofunctionalization steps with different ssDNA sequences. By employing a rapid and efficient drop and collect protocol, the specific detection of 1 pg/µL ssDNA Brett of a short sequence and 100 ng/μL DNA of B. bruxellensis extracted from a wine sample (VR2008) is reported.
Complete list of metadata

https://hal-utt.archives-ouvertes.fr/hal-03619677
Contributor : Rodica Elena Ionescu Connect in order to contact the contributor
Submitted on : Friday, March 25, 2022 - 11:42:11 AM
Last modification on : Sunday, June 26, 2022 - 9:32:45 AM

Links full text

Identifiers

Collections

CNRS | UTT

Citation

Simone Poggesi, Lan Zhou, Giuliocesare Casari Bariani, Rakesh Mittapalli, Marisa Manzano, et al.. Quartz Crystal Microbalance Genosensing of Brettanomyces bruxellensis Yeast in Wine Using a Rapid and Efficient Drop and Collect Protocol. Crystals, MDPI, 2021, 11 (5), pp.562. ⟨10.3390/cryst11050562⟩. ⟨hal-03619677⟩

Share

Metrics

Record views

19