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Membrane-substrate separation distance assessed by normalized total internal reflection fluorescence microscopy

Abstract : As a consequence of the recent progress in nanoscale technology, more and more sensitive methods are developed to characterize and understand the dynamic of cell membrane adhesion process. In this paper we present a new quantitative method to measure the separation distances between the membrane and the substrate. This technique is based on a normalization of Total Internal Reflection Fluorescence (TIRF) images by usual epi-illumination images. This simple method allows to achieve a nanometric axial resolution, typically 10 nm. We demonstrate the potential of our technique through the study of phospholipids membranes such as Giant Unilamellar Vesicles (GUVs), which are usual biomimetic systems to investigate membrane-substrate interactions.
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Conference papers
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https://hal-utt.archives-ouvertes.fr/hal-02531225
Contributor : Jean-Baptiste Vu Van <>
Submitted on : Friday, April 3, 2020 - 3:17:17 PM
Last modification on : Saturday, April 4, 2020 - 1:44:45 AM

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Marcelina Cardoso dos Santos, Cyrille Vézy, Rodolphe Jaffiol. Membrane-substrate separation distance assessed by normalized total internal reflection fluorescence microscopy. SPIE BiOS, Feb 2014, San Francisco, United States. pp.89491H, ⟨10.1117/12.2037280⟩. ⟨hal-02531225⟩

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