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Conference Papers Year : 2016

Vesicle adhesion depicted at nanoscale with normalized total internal reflection fluorescence microscopy

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Abstract

We propose a novel fluorescence microscopy technique to study the adhesion of Giant Unilamellar Vesicles. Their adhesion is assessed through the normalization of Total Internal Reflection Fluorescence (TIRF) images by epi-fluorescence ones. This can be achieved by using a motorized rotatable mirror to switch from epi-fluorescence to TIRF. This method allows us to study the vesicle adhesion from ultra-weak to strong membrane surface interactions and to measure the absolute distance between the vesicles and various chemically functionalized glass substrates at the nanoscale.
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hal-02531206 , version 1 (03-04-2020)

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Marcelina Cardoso dos Santos, Cyrille Vézy, Rodolphe Jaffiol. Vesicle adhesion depicted at nanoscale with normalized total internal reflection fluorescence microscopy. SPIE BiOS, Feb 2016, San Francisco, United States. pp.97190I, ⟨10.1117/12.2208429⟩. ⟨hal-02531206⟩

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