 |
Conference papers
Vesicle adhesion depicted at nanoscale with normalized total internal reflection fluorescence microscopy
Abstract : We propose a novel fluorescence microscopy technique to study the adhesion of Giant Unilamellar Vesicles. Their adhesion is assessed through the normalization of Total Internal Reflection Fluorescence (TIRF) images by epi-fluorescence ones. This can be achieved by using a motorized rotatable mirror to switch from epi-fluorescence to TIRF. This method allows us to study the vesicle adhesion from ultra-weak to strong membrane surface interactions and to measure the absolute distance between the vesicles and various chemically functionalized glass substrates at the nanoscale.
https://hal-utt.archives-ouvertes.fr/hal-02531206
Contributor : Jean-Baptiste Vu Van Connect in order to contact the contributor
Submitted on : Friday, April 3, 2020 - 3:08:32 PM
Last modification on : Wednesday, September 15, 2021 - 3:03:56 PM
Contributor : Jean-Baptiste Vu Van Connect in order to contact the contributor
Submitted on : Friday, April 3, 2020 - 3:08:32 PM
Last modification on : Wednesday, September 15, 2021 - 3:03:56 PM