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Vesicle adhesion depicted at nanoscale with normalized total internal reflection fluorescence microscopy

Abstract : We propose a novel fluorescence microscopy technique to study the adhesion of Giant Unilamellar Vesicles. Their adhesion is assessed through the normalization of Total Internal Reflection Fluorescence (TIRF) images by epi-fluorescence ones. This can be achieved by using a motorized rotatable mirror to switch from epi-fluorescence to TIRF. This method allows us to study the vesicle adhesion from ultra-weak to strong membrane surface interactions and to measure the absolute distance between the vesicles and various chemically functionalized glass substrates at the nanoscale.
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Conference papers
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https://hal-utt.archives-ouvertes.fr/hal-02531206
Contributor : Jean-Baptiste Vu Van <>
Submitted on : Friday, April 3, 2020 - 3:08:32 PM
Last modification on : Saturday, April 4, 2020 - 1:44:51 AM

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P2MN | UTT | CNRS

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Marcelina Cardoso dos Santos, Cyrille Vézy, Rodolphe Jaffiol. Vesicle adhesion depicted at nanoscale with normalized total internal reflection fluorescence microscopy. SPIE BiOS, Feb 2016, San Francisco, United States. pp.97190I, ⟨10.1117/12.2208429⟩. ⟨hal-02531206⟩

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