Simple approaches for designing controlled plasmonic substrates and their use in biological applications
Abstract
Interesting “controlled” dispersion of gold/silver nano-structures (NPs) are obtained onto glass substrates when using high annealing temperatures. Such supports proved stable plasmonic properties being very good candidates for designing localized surface plasmon resonance (LSPR) immunosensors. For specific and local optical investigations, several areas containing nanostructures are easily analyzed and compared thanks to an initially graphed of a micro electron transmission grid (TEM-grid) or/and a metal-stapler onto a solid support. Moreover, facile multiplexing “bar-code” samples containing nanoparticles are created by using a scotch – tape approach to simply delimitate different NPs-areas on a given glass support. (Bio)functionalizations steps of various micro-areas containing annealed nanoparticles are investigated for their plasmonic extinction characteristics – peak intensity and resonant maximum wavelength position- in the case of proteins and herbicides detection.