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Poster communications

Nanoscale topography of cells revealed by variable-angle Total Internal Reflection Fluorescence Microscopy (vaTIRFM)

Abstract : We propose an improved version of variable-angle total internal reflection fluorescence microscopy (vaTIRFM) adapted to modern TIRF setup. This technique involves the recording of a stack of TIRF images, by gradually increasing the incident angle of the light beam on the sample. A comprehensive theory was developed to extract the membrane/substrate separation distance from fluorescently labeled cell membranes. A straightforward image processing was then established to compute the topography of cells with a nanometric axial resolution, typically 10–20 nm. To highlight the new opportunities offered by vaTIRFM to quantify adhesion process of motile cells, adhesion of MDA-MB-231 cancer cells on glass substrate coated with fibronectin was examined.
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Poster communications
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https://hal-utt.archives-ouvertes.fr/hal-02460110
Contributor : Jean-Baptiste Vu Van <>
Submitted on : Wednesday, January 29, 2020 - 6:59:04 PM
Last modification on : Wednesday, February 5, 2020 - 3:44:02 PM

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  • HAL Id : hal-02460110, version 1

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P2MN | UTT | CNRS

Citation

Dalia El Arawi, Marcelina Cardoso dos santos, Régis Déturche, Cyrille Vézy, Rodolphe Jaffiol. Nanoscale topography of cells revealed by variable-angle Total Internal Reflection Fluorescence Microscopy (vaTIRFM). Focus on microscopy 2017, Apr 2017, Bordeaux, France. ⟨hal-02460110⟩

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