Surface enhanced Raman spectroscopy phylogenetictree for genosensing of Brettanomyces bruxellensis yeast on nanostructured ultrafine glass supports

Abstract : Novel genosensors (Brett DNA) on nanostructured ultrafine coverslips are reported for the identification of Brettanomyces bruxellensis yeast that is well-known to create complex and difficult problems in aged red wine all over the world. Surface Enhanced Raman Scattering (SERS) and Localize Surface Plasmon Resonance (LSPR) are used as detection methods of target-ssDNA yeast. A specific detection is obtained in the presence of thiol-DNA probe. SERS technique made possible the detection of Brett-DNA with a limit of detection (LD) of 0.1 ng/μL((57.2 nM) and with a wide dynamic range (DR), while LSPR revealed a DL of 0,5 ng/μL real DNA extracted from Brettanomyces bruxellensis DKA in the laboratory and a limited DR (0.5 ng/μL to 5 ng/μL). In this work, fourteen yeasts that can be found in wine-growing environments were SERS tested to confirm the selectivity of the proposed Brett-DNA sensing schemes and used in the construction of the first SERS phylogenetic tree.