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Journal articles
Surface Enhanced Raman Spectroscopy detection of Brettanomyces bruxellensis yeast on nanostructured ultrafine glass supports
Abstract : Novel genosensors (Brett DNA) on nanostructured ultrafine coverslips are reported for the identification of Brettanomyces bruxellensis yeast that is well-known to create complex and difficult problems in aged red wine all over the word. Surface Enhanced Raman Scattering (SERS) and Localize Surface Plasmon Resonance (LSPR) are used as detection methods of target-ssDNA yeast. A specific detection is obtained in the presence of thiol-DNA probe. SERS technique made possible the detection of Brett-DNA with a limit of detection (LD) of 0,1 ng/μL((57,2 nM) and with a wide dynamic range (DR), while LSPR revealed a DL of 0,5 ng/μL real DNA extracted from Brettanomyces bruxellensis DKA in the laboratory and a limited DR (0.5 ng/ μL to 5 ng/ μL). In this work, 14 yeasts that can be found in wine-growing environments were SERS tested to confirm the selectivity of the proposed Brett-DNA sensing schemes and used in the construction of the first SERS phylogenetic tree.
https://hal-utt.archives-ouvertes.fr/hal-02391604
Contributor : Jean-Baptiste Vu Van Connect in order to contact the contributor
Submitted on : Tuesday, December 3, 2019 - 4:05:43 PM
Last modification on : Wednesday, September 15, 2021 - 3:03:44 PM
Contributor : Jean-Baptiste Vu Van Connect in order to contact the contributor
Submitted on : Tuesday, December 3, 2019 - 4:05:43 PM
Last modification on : Wednesday, September 15, 2021 - 3:03:44 PM
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