Microfluidity mapping using fluorescence correlation spectroscopy: A new way to investigate plasma membrane microorganization of living cells

Pascale Winckler (1) , Aurélie Cailler (1) , Régis Deturche (1) , Pierre Jeannesson (2) , Hamid Morjani (3) , Rodolphe Jaffiol (1)

1 LNIO - Laboratoire de Nanotechnologie et d'Instrumentation Optique
2 MEDyC - Matrice Extracellulaire et Dynamique Cellulaire - UMR CNRS 7369
3 Unite Medicament Dynam Intracellulaire Architectu, CNRS, UFR Pharm

Diffusion time distribution analysis has been employed to highlight the microfluidity fingerprint of plasma membrane of living cells. Diffusion time measurements were obtained through fluorescence correlation spectroscopy performed at the single cell level, over various eukaryotic cell lines (MCF7, LR73, KB3.1, MESSA and MDCKII). The nonsymmetric profile of the diffusion time distributions established experimentally, is discussed according to Monte Carlo simulations, which reproduce the diffusion of the fluorescent probe in heterogeneous membrane.

Mots clés: Microdomain Membrane fluidity; Plasma membrane organization; Fluorescence spectroscopy; Fluorescence correlation spectroscopy

Domaines: Biochimie [q-bio.BM]; Biophysique

Pascale Winckler, Aurélie Cailler, Régis Deturche, Pierre Jeannesson, Hamid Morjani, et al.. Microfluidity mapping using fluorescence correlation spectroscopy: A new way to investigate plasma membrane microorganization of living cells. Biochimica et Biophysica Acta:Biomembranes, 2012, 1818 (11), pp.2477-2485. ⟨10.1016/j.bbamem.2012.05.018⟩. ⟨hal-02383196⟩