Microfluidity mapping using fluorescence correlation spectroscopy: A new way to investigate plasma membrane microorganization of living cells - Archive ouverte HAL Access content directly
Journal Articles Biochimica et Biophysica Acta:Biomembranes Year : 2012

Microfluidity mapping using fluorescence correlation spectroscopy: A new way to investigate plasma membrane microorganization of living cells

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Abstract

Diffusion time distribution analysis has been employed to highlight the microfluidity fingerprint of plasma membrane of living cells. Diffusion time measurements were obtained through fluorescence correlation spectroscopy performed at the single cell level, over various eukaryotic cell lines (MCF7, LR73, KB3.1, MESSA and MDCKII). The nonsymmetric profile of the diffusion time distributions established experimentally, is discussed according to Monte Carlo simulations, which reproduce the diffusion of the fluorescent probe in heterogeneous membrane.

Dates and versions

hal-02383196 , version 1 (27-11-2019)

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Pascale Winckler, Aurélie Cailler, Régis Deturche, Pierre Jeannesson, Hamid Morjani, et al.. Microfluidity mapping using fluorescence correlation spectroscopy: A new way to investigate plasma membrane microorganization of living cells. Biochimica et Biophysica Acta:Biomembranes, 2012, 1818 (11), pp.2477-2485. ⟨10.1016/j.bbamem.2012.05.018⟩. ⟨hal-02383196⟩
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