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Journal Articles Biosensors Year : 2018

Microwave spectroscopic detection of human Hsp70 protein on annealed gold nanostructures on ITO glass strips

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Abstract

Conductive indium-tin oxide (ITO) and non-conductive glass substrates were successfully modified with embedded gold nanoparticles (AuNPs) formed by controlled thermal annealing at 550 °C for 8 h in a preselected oven. The authors characterized the formation of AuNPs using two microscopic techniques: scanning electron microscopy (SEM) and atomic force microscopy (AFM). The analytical performances of the nanostructured-glasses were compared regarding biosensing of Hsp70, an ATP-driven molecular chaperone. In this work, the human heat-shock protein (Hsp70), was chosen as a model biomarker of body stress disorders for microwave spectroscopic investigations. It was found that microwave screening at 4 GHz allowed for the first time the detection of 12 ng/µL/cm2 of Hsp70.

Dates and versions

hal-02365657 , version 1 (15-11-2019)

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Rodica Elena Ionescu, Raphael Selon, Nicolas Pocholle, Lan Zhou, Anna Rumyantseva, et al.. Microwave spectroscopic detection of human Hsp70 protein on annealed gold nanostructures on ITO glass strips. Biosensors, 2018, 8 (4), pp.1-10. ⟨10.3390/bios8040118⟩. ⟨hal-02365657⟩
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