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Nanometer-Scale Resolution Achieved with Nonradiative Excitation

Abstract : Nonradiative Excitation Fluorescence Microscopy (NEFM) is a promising technique allowing the observation of biological samples beyond the diraction limit. By coating a substrate with an homogeneous monolayer of quantum dots (QDs), NEFM is achieved through a nonradiative energy transfer from QDs (donors) to dye molecules located in the sample (acceptors). The excitation depth of the sample is then given by the För-ster radius, which corresponds to few nanometers above the surface. The powerful axial resolution of NEFM is highlighted by observing the adhesion of Giant Unilamellar Vesi-cles (GUVs) on strong interaction with coated surfaces. In this paper, we demonstrate that the QD-quenching level is valuable to calculate and map the distance between the membrane and the surface with a high precision along the optical axis. By tuning the electrostatic interactions between the membrane and the substrate, we have been able to measure a height displacement of ≈ 1 nm of the lipid membrane. The experimental results were discussed according to simulations, which take into account all the common forces appearing between lipid membranes and surfaces.
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Lina Riachy, Dalia El Arawi, Rodolphe Jaffiol, Cyrille Vézy. Nanometer-Scale Resolution Achieved with Nonradiative Excitation. ACS photonics, American Chemical Society,, 2018, 5 (6), pp.2217-2224. ⟨10.1021/acsphotonics.8b00063⟩. ⟨hal-02363346⟩



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