Electrochemical lateral flow immunosensor for detection and quantification of dengue NS1 protein

Prima Dewi Sinawang 1 Varun Rai 1 Rodica Elena Ionescu 2, 1 Robert Marks 3, 1
1 School of Materials Science and Engineering [Singapore]
2 LNIO - Laboratoire de Nanotechnologie et d'Instrumentation Optique
ICD - Institut Charles Delaunay
3 BGU - Ben-Gurion University of the Negev
Abstract : An Electrochemical Lateral Flow Immunosensor (ELFI) is developed combining screen-printed gold electrodes (SPGE) enabling quantification together with the convenience of a lateral flow test strip. A cellulose glassy fiber paper conjugate pad retains the marker immunoelectroactive nanobeads which will bind to the target analyte of interest. The specific immunorecognition event continues to occur along the lateral flow bed until reaching the SPGE-capture antibodies at the end of the cellulosic lateral flow strip. The rationale of the immunoassay consists in the analyte antigen NS1 protein being captured selectively and specifically by the dengue NS1 antibody conjugated onto the immunonanobeads thus forming an immunocomplex. With the aid of a running buffer, the immunocomplexes flow and reach the immuno-conjugated electrode surface and form specific sandwich-type detection due to specific, molecular recognition, while unbound beads move along past the electrodes. The successful sandwich immunocomplex formation is then recorded electrochemically. Specific detection of NS1 is translated into an electrochemical signal contributed by a redox label present on the bead-immobilized detection dengue NS1 antibody while a proportional increase of faradic current is observed with increase in analyte NS1 protein concentration. The first generation ELFI prototype is simply assembled in a cassette and successfully demonstrates wide linear range over a concentration range of 1–25 ng/mL with an ultrasensitive detection limit of 0.5 ng/mL for the qualitative and quantitative detection of analyte dengue NS1 protein.
Keywords : Cellulose paper Gold nanoparticles (AuNPs) Electrochemical impedance spectroscopy (EIS) Electrochemical lateral flow immunosensor Dengue virus DENV NS1 protein Cyclic voltammetry
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Journal articles
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https://hal-utt.archives-ouvertes.fr/hal-02309921
Contributor : Daniel Gavrysiak <>
Submitted on : Wednesday, October 9, 2019 - 4:40:35 PM
Last modification on : Thursday, October 10, 2019 - 1:32:58 AM

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Prima Dewi Sinawang, Varun Rai, Rodica Elena Ionescu, Robert Marks. Electrochemical lateral flow immunosensor for detection and quantification of dengue NS1 protein. Biosensors and Bioelectronics, Elsevier, 2016, 77, pp.400-408. ⟨10.1016/j.bios.2015.09.048⟩. ⟨hal-02309921⟩

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