Biopolymers phase separation monitored by a plasmonic sensor

Suzanna Akil-Jradi (1) , Safi Jradi (1) , Jerome Plain (1) , Jean-Louis Bijeon (1) , Christian Sanchez (2) , Renaud Bachelot (1) , Pascal Royer (1)

1 LNIO - Laboratoire de Nanotechnologie et d'Instrumentation Optique
2 UMR IATE - Ingénierie des Agro-polymères et Technologies Émergentes

We report here a real-time study of interactions induced phase separation between β-lactoglobulin (BLG) and Acacia gum (AG) by analyzing the localized surface plasmon resonance of silver nanoparticles. We showed that the binding of BLG to AG is accompanied by refractive index changes, in relation with optical properties and structural changes of the complexes formed.

Keywords: acacia gum; beta-lactoglobulin; complex coacervation; interactions; ordering kinetics; light-scattering; surface; resonance; nanoparticle; ph

Keywords: protein

Domains: Engineering Sciences [physics]; Micro and nanotechnologies/Microelectronics; Chemical Sciences; Polymers; Engineering Sciences [physics]; Optics / Photonic

Suzanna Akil-Jradi, Safi Jradi, Jerome Plain, Jean-Louis Bijeon, Christian Sanchez, et al.. Biopolymers phase separation monitored by a plasmonic sensor. Chemical Communications, 2011, 47 (8), pp.2444-2446. ⟨10.1039/c0cc05212d⟩. ⟨hal-02296790⟩